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SEPARATION OF HUMAN LYMPHOID CELLS INTO G(1), S, AND G(2) CELL CYCLE POPULATIONS BY USE OF A VELOCITY SEDIMENTATION TECHNIQUE

Human lymphoid tissue culture cells can be separated according to cell size and corresponding cell cycle phase with a velocity sedimentation centrifugation method employing a continuous 5–20% wt/wt Ficoll gradient. A 7-fold increase in streaming limit was achieved by placing a buffer zone of isosmol...

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Bibliografske podrobnosti
Main Authors: Everson, Lloyd K., Buell, Donald N., Rogentine, G. Nicholas
Format: Artigo
Jezik:Inglês
Izdano: The Rockefeller University Press 1973
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2139489/
https://ncbi.nlm.nih.gov/pubmed/4568300
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