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Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase.

Restriction fragments containing the gene encoding cyanase, cynS, without its transcriptional regulatory sequences were placed downstream of lac and tac promoters in various pUC derivatives to maximize production of cyanase. Plasmid pSJ105, which contains the cynS gene and an upstream open reading f...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Sung, Y C, Anderson, P M, Fuchs, J A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1987
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213930/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2822670/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.11.5224-5230.1987
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