Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens.

The virD locus of Agrobacterium tumefaciens Ti plasmid pTiA6 was sequenced. Computer analysis of the sequence indicated five possible open reading frames (ORFs) within this locus. Two additional ORFs were identified distal to this locus. However, only two polypeptides of apparent molecular masses 16...

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Εκδόθηκε σε:	J Bacteriol
Κύριοι συγγραφείς:	Jayaswal, R K, Veluthambi, K, Gelvin, S B, Slightom, J L
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	American Society for Microbiology (ASM) 1987
Θέματα:	Research Article
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213905/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2822660/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.11.5035-5045.1987
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