Exotoxin A of Pseudomonas aeruginosa: active, cloned toxin is secreted into the periplasmic space of Escherichia coli.

We subcloned the structural gene for exotoxin A (ETA) of Pseudomonas aeruginosa in front of the tac promoter in an Escherichia coli expression vector and studied the intracellular location and properties of the protein product. The E. coli K-12 strain that carried this recombinant plasmid produced a...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Douglas, C M, Guidi-Rontani, C, Collier, R J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1987
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213893/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3117768/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.11.4962-4966.1987
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