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LiCl treatment releases a nickase implicated in genetic transformation of Streptococcus pneumoniae.

When cells competent for genetic transformation of Streptococcus pneumoniae which could bind and enable entry of extracellular DNA molecules were treated with LiCl, they released a nickase that introduced nicks into a double-stranded DNA in the presence of EDTA. The nickase was specific for competen...

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Détails bibliographiques
Publié dans:J Bacteriol
Auteurs principaux: Fujii, T, Naka, D, Toyoda, N, Seto, H
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1987
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213883/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2822653/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.11.4901-4906.1987
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