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Cloning of the debranching-enzyme gene from Thermoanaerobium brockii into Escherichia coli and Bacillus subtilis.

The gene for an enzyme with single or dual specificity on complex carbohydrates has been transferred from its native host (Thermoanaerobium brockii), a thermophilic anaerobe, into Escherichia coli and Bacillus subtilis. Most of the gene coding region is in a 2.2-kilobase PstI fragment that is common...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Coleman, R D, Yang, S S, McAlister, M P
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1987
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213744/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3040687/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.9.4302-4307.1987
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