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MOLECULAR GROWTH REQUIREMENTS OF SINGLE MAMMALIAN CELLS : III. QUANTITATIVE COLONIAL GROWTH OF SINGLE S3 CELLS IN A MEDIUM CONTAINING SYNTHETIC SMALL MOLECULAR CONSTITUENTS AND TWO PURIFIED PROTEIN FRACTIONS

Two purified serum protein fractions, fetuin and serum albumin, will replace whole or dialyzed serum in supporting the growth of single S3 HeLa cells in an otherwise chemically defined nutrient solution. In the serum-free medium, single S3 cells will form macroscopic colonies with essentially 100 pe...

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Bibliografiske detaljer
Main Authors: Fisher, Harold W., Puck, Theodore T., Sato, Gordon
Format: Artigo
Sprog:Inglês
Udgivet: The Rockefeller University Press 1959
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2136981/
https://ncbi.nlm.nih.gov/pubmed/13654634
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