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CLONAL GROWTH OF MAMMALIAN CELLS IN VITRO : GROWTH CHARACTERISTICS OF COLONIES FROM SINGLE HELA CELLS WITH AND WITHOUT A "FEEDER" LAYER

Two methods for simple and rapid plating of single HeLa cells, human, carcinomatous cells, are described. These result in growth and formation of colonies from each single cell. One of these procedures uses irradiated, non-multiplying "feeder" cells to condition the medium. The second requ...

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Autors principals: Puck, Theodore T., Marcus, Philip I., Cieciura, Steven J.
Format: Artigo
Idioma:Inglês
Publicat: The Rockefeller University Press 1956
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2136583/
https://ncbi.nlm.nih.gov/pubmed/13286432
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