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Cloning and expression of the Acinetobacter calcoaceticus mutarotase gene in Escherichia coli.

This article describes the cloning of the mutarotase gene from Acinetobacter calcoaceticus and its expression in Escherichia coli. Purification of mutarotase (EC 5.1.3.3) led to a single polypeptide of 40 kilodaltons. The sequences of 27 N-terminal and 76 C-terminal amino acids were determined. From...

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Autores principales: Gatz, C, Altschmied, J, Hillen, W
Formato: Artigo
Lenguaje:Inglês
Publicado: 1986
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC213416/
https://ncbi.nlm.nih.gov/pubmed/3531172
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