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Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number.

The trfA gene of the broad-host-range plasmid RK2 is essential for initiation of plasmid replication. Two related TrfA proteins of 43 and 32 kilodaltons (kDa) are produced by independent translation initiation at two start codons within the trfA open reading frame. These proteins were o overproduced...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Durland, R H, Helinski, D R
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213366/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2193920/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.7.3849-3858.1990
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