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Insertional mutagenesis of Listeria monocytogenes with a novel Tn917 derivative that allows direct cloning of DNA flanking transposon insertions.

To carry out efficient insertional mutagenesis in Listeria monocytogenes and to facilitate the characterization of disrupted genes, two novel derivatives of Tn917 were constructed, Tn917-LTV1 and Tn917-LTV3. The derivatives (i) transpose at a significantly elevated frequency, (ii) generate transcrip...

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Udgivet i:J Bacteriol
Principais autores: Camilli, A, Portnoy, A, Youngman, P
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1990
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213352/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2163385/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.7.3738-3744.1990
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