A Novel In Vivo Assay Reveals Inhibition of Ribosomal Nuclear Export in Ran-Cycle and Nucleoporin Mutants

To identify components involved in the nuclear export of ribosomes in yeast, we developed an in vivo assay exploiting a green fluorescent protein (GFP)-tagged version of ribosomal protein L25. After its import into the nucleolus, L25-GFP assembles with 60S ribosomal subunits that are subsequently ex...

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Bibliographic Details
Main Authors: Hurt, Ed, Hannus, Stefan, Schmelzl, Birgit, Lau, Denise, Tollervey, David, Simos, George
Format: Artigo
Language:Inglês
Published: The Rockefeller University Press 1999
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2132911/
https://ncbi.nlm.nih.gov/pubmed/9971735
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