Purification, characterization, and in vivo reconstitution of Klebsiella aerogenes urease apoenzyme.

Urease was purified from recombinant Klebsiella aerogenes which was grown in the absence of nickel. The protein was inactive and contained no transition metals, yet it possessed the same heteropolymeric structure as native enzyme, demonstrating that Ni is not required for intersubunit association. N...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Lee, M H, Mulrooney, S B, Hausinger, R P
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213271/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2142939/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.8.4427-4431.1990
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