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Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthemi EC16.

The pehX gene encoding extracellular exo-poly-alpha-D-galacturonosidase (exoPG; EC 3.2.1.82) was isolated from a genomic library of the pectate lyase-deficient Erwinia chrysanthemi mutant UM1005 (a Nalr Kanr delta pelABCE derivative of EC16) by immunoscreening 2,800 Escherichia coli HB101 transforma...

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Bibliographic Details
Main Authors: He, S Y, Collmer, A
Format: Artigo
Language:Inglês
Published: 1990
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC213154/
https://ncbi.nlm.nih.gov/pubmed/2168372
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