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Mutations that alter the equilibrium between open and closed conformations of Escherichia coli maltose-binding protein impede its ability to enhance the solubility of passenger proteins

Certain highly soluble proteins, such as Escherichia coli maltose-binding protein (MBP), have the ability to enhance the solubility of their fusion partners, making them attractive vehicles for the production of recombinant proteins, yet the mechanism of solubility enhancement remains poorly underst...

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Main Authors: Nallamsetty, Sreedevi, Waugh, David S.
Formato: Artigo
Idioma:Inglês
Publicado: 2007
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2129132/
https://ncbi.nlm.nih.gov/pubmed/17964542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2007.10.060
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