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Vector for regulated expression of cloned genes in a wide range of gram-negative bacteria.

A pKT231-based broad-host-range plasmid vector was constructed which enabled regulation of expression of cloned genes in a wide range of gram-negative bacteria. This vector, pNM185, contained upstream of its EcoRI, SstI, and SstII cloning sites the positively activated pm twin promoters of the TOL p...

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Autors principals: Mermod, N, Ramos, J L, Lehrbach, P R, Timmis, K N
Format: Artigo
Idioma:Inglês
Publicat: 1986
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC212908/
https://ncbi.nlm.nih.gov/pubmed/3525513
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