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Synthesis of linear plasmid multimers in Escherichia coli K-12.

Linear plasmid multimers were identified in extracts of recB21 recC22 strains containing derivatives of the ColE1-type plasmids pACYC184 and pBR322. A mutation in sbcB increases the proportion of plasmid DNA as linear multimers. A model to explain this is based on proposed roles of RecBC enzyme and...

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Bibliographic Details
Main Authors: Cohen, A, Clark, A J
Format: Artigo
Language:Inglês
Published: 1986
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC212879/
https://ncbi.nlm.nih.gov/pubmed/3522548
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