Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis.

A Bacillus subtilis 2.7-kilobase DNA fragment containing an intracellular protease gene was cloned into Escherichia coli. The transformants produced an intracellular protease of approximately 35,000 Mr whose activity was inhibited by both phenylmethylsulfonyl fluoride and EDTA. Introduction of the f...

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Bibliographic Details
Main Authors: Koide, Y, Nakamura, A, Uozumi, T, Beppu, T
Format: Artigo
Language:Inglês
Published: 1986
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC212848/
https://ncbi.nlm.nih.gov/pubmed/3087947
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