Positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius by use of 5-fluoroorotic acid.

Uracil auxotrophs of Sulfolobus acidocaldarius were positively selected by using 5-fluoroorotic acid. The wild-type strain was unable to grow in medium containing 5-fluoroorotic acid, whereas the mutants grew normally. Positive selection could be done for the auxotrophs. Mutants deficient in orotidi...

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Main Authors: Kondo, S, Yamagishi, A, Oshima, T
Format: Artigo
Jezik:Inglês
Izdano: 1991
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC212540/
https://ncbi.nlm.nih.gov/pubmed/1938966
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