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Construction of an IS946-based composite transposon in Lactococcus lactis subsp. lactis.

An artificial composite transposon was constructed based on the lactococcal insertion sequence IS946. A 3.0-kb element composed of the pC194 cat gene (Cmr) flanked by inversely repeated copies of IS946 was assembled on pBluescript KS+. When subcloned into the shuttle vector pSA3 (Emr), two putative...

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Detalles Bibliográficos
Publicado en:J Bacteriol
Principais autores: Romero, D A, Klaenhammer, T R
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1991
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212528/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1657893/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.23.7599-7606.1991
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