Expression of the cloned coliphage T3 S-adenosylmethionine hydrolase gene inhibits DNA methylation and polyamine biosynthesis in Escherichia coli.

We have developed a new research tool for the study of S-adenosylmethionine (AdoMet) metabolism by cloning the coliphage T3 AdoMet hydrolase (AdoMetase; EC 3.3.1.2) gene into the M13mp8 expression vector. The recombinant bacteriophage clones expressed an AdoMetase activity in Escherichia coli like t...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Hughes, J A, Brown, L R, Ferro, A J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1987
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212442/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3301808/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.8.3625-3632.1987
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