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Molecular cloning and characterization of a Streptococcus sanguis DNase necessary for repair of DNA damage induced by UV light and methyl methanesulfonate.

We developed a method for cloning cellular nucleases from streptococci. Recombinant lambda gt11 bacteriophage containing streptococcal nuclease determinants were identified by the production of pink plaques on toluidine blue O DNase plates. We used this technique to clone a 3.2-kilobase-pair EcoRI f...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Lindler, L E, Macrina, F L
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1987
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212370/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3036775/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.7.3199-3208.1987
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