Use of on-section immunolabeling and cryosubstitution for studies of bacterial DNA distribution.

Escherichia coli cells were very rapidly frozen and substituted at a low temperature with 3% glutaraldehyde in acetone. Infiltration and embedding with Lowicryl K4M were carried out at -35 degrees C. This procedure resulted in good structural preservation of both the nucleoid morphology and its DNA...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Hobot, J A, Bjornsti, M A, Kellenberger, E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1987
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212088/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3553155/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.5.2055-2062.1987
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