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A broad-host-range expression vector based on the pL promoter of coliphage lambda: regulated synthesis of human interleukin 2 in Erwinia and Serratia species.

We report the construction of a broad-host-range expression vector based on an RSF1010-derived replicon. The vector carries the strong leftward promoter (pL) of coliphage lambda as well as the cI857 allele, which codes for a thermolabile repressor protein. The coding region of mature human interleuk...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Leemans, R, Remaut, E, Fiers, W
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1987
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212042/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2952635/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.5.1899-1904.1987
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