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Molecular cloning, characterization, and chromosomal localization of dapF, the Escherichia coli gene for diaminopimelate epimerase.

The Escherichia coli dapF gene was isolated from a cosmid library as a result of screening for clones overproducing diaminopimelate epimerase. Insertional mutagenesis was performed on the cloned dapF gene with a mini-Mu transposon, leading to chloramphenicol resistance. One of these insertions was t...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Richaud, C, Higgins, W, Mengin-Lecreulx, D, Stragier, P
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1987
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211967/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3031013/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.4.1454-1459.1987
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