Efficient transfection of the archaebacterium Halobacterium halobium.

We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 X 10(7) transfectants per microgram of DNA. Betw...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Cline, S W, Doolittle, W F
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1987
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211943/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3818549/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.3.1341-1344.1987
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