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Purification and use of Methanobacterium wolfei pseudomurein endopeptidase for lysis of Methanobacterium thermoautotrophicum.

The pseudomurein-degrading enzyme from autolysates of Methanobacterium wolfei was purified approximately 500-fold to electrophoretic homogeneity by ion-exchange chromatography under anaerobic conditions. Analysis of the soluble cell wall fragments produced by the pure enzyme from a cell wall prepara...

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Dettagli Bibliografici
Autori principali: Kiener, A, König, H, Winter, J, Leisinger, T
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1987
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC211894/
https://ncbi.nlm.nih.gov/pubmed/3546261
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