EnvZ, a transmembrane environmental sensor of Escherichia coli K-12, is phosphorylated in vitro.
By fusing the transcriptional and translational start signals of lacZ to envZ, we have obtained high-level synthesis of a truncated EnvZ protein (EnvZ115) in which the first 38 amino acids of EnvZ are replaced with the first 8 amino acids of LacZ. Using this construct, we have partially purified the...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1988
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211718/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3056929/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.12.5971-5973.1988 |
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