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Isolation and characterization of Mycoplasma mycoides subsp. mycoides mutants deficient in nucleoside monophosphate transport.

To select mutants lacking dAMP uptake, log-phase cells of Mycoplasma mycoides subsp. mycoides Y were incubated with high-specific-activity [32P]dAMP and then stored several weeks at -20 degrees C to allow 32P decay before plating out. Mutants were screened for lack of labeling by [32P]dAMP. Two muta...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Youil, R, Finch, L R
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211704/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3056924/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.12.5922-5924.1988
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