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Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors

We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to study biochemical events in the transport of macromolecules across the nuclear envelope. While treatment of cultured cells with digitonin permeabilizes the plasma membranes to macromolecules, the nuclear envel...

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Bibliografske podrobnosti
Format: Artigo
Jezik:Inglês
Izdano: The Rockefeller University Press 1990
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2116268/
https://ncbi.nlm.nih.gov/pubmed/2391365
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