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A chimeric mitochondrial precursor protein with internal disulfide bridges blocks import of authentic precursors into mitochondria and allows quantitation of import sites
Bovine pancreatic trypsin inhibitor (which contains three intramolecular disulfide bridges) was chemically coupled to the COOH terminus of a purified artificial mitochondrial precursor protein. When the resulting chimeric precursor was presented to energized isolated yeast mitochondria, its trypsin...
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| Formato: | Artigo |
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| Idioma: | Inglês |
| Publicado em: |
The Rockefeller University Press
1988
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2115651/ https://ncbi.nlm.nih.gov/pubmed/2904445 |
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