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Isolation of Chinese hamster ovary cell lines temperature conditional for the cell-surface expression of integral membrane glycoproteins
A procedure is described to select mutants of Chinese hamster ovary cells that are conditionally defective for the cell-surface expression of integral membrane glycoproteins, including the hemagglutinin (HA) of influenza virus. Using a combination of cell sorting and biochemical screening, seven cel...
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| Format: | Artigo |
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| Sprog: | Inglês |
| Udgivet: |
The Rockefeller University Press
1989
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2115425/ https://ncbi.nlm.nih.gov/pubmed/2537314 |
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