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The mechanism of anaphase spindle elongation: uncoupling of tubulin incorporation and microtubule sliding during in vitro spindle reactivation
To study tubulin polymerization and microtubule sliding during spindle elongation in vitro, we developed a method of uncoupling the two processes. When isolated diatom spindles were incubated with biotinylated tubulin (biot-tb) without ATP, biot-tb was incorporated into two regions flanking the zone...
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| Formato: | Artigo |
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| Idioma: | Inglês |
| Publicado em: |
The Rockefeller University Press
1988
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2115210/ https://ncbi.nlm.nih.gov/pubmed/3047143 |
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