Isolation, hyperexpression, and sequencing of the aceA gene encoding isocitrate lyase in Escherichia coli.
A structural gene for isocitrate lyase was isolated from a cosmid containing an ace locus of the Escherichia coli chromosome. Cloning and expression under control of the tac promoter in a multicopy plasmid showed that a 1.7-kilobase-pair DNA segment was sufficient for complementation of an aceA dele...
Saved in:
| Published in: | J Bacteriol |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1988
|
| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211486/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3049537/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.10.4528-4536.1988 |
| Tags: |
No Tags, Be the first to tag this record!
|