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Microinjection of ubiquitin: changes in protein degradation in HeLa cells subjected to heat-shock
Ubiquitin was radiolabeled by reaction with 125I-Bolton-Hunter reagent and introduced into HeLa cells using erythrocyte-mediated microinjection. The injected cells were then incubated at 45 degrees C for 5 min (reversible heat-shock) or for 30 min (lethal heat-shock). After either treatment, there w...
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| Formato: | Artigo |
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| Idioma: | Inglês |
| Publicado em: |
The Rockefeller University Press
1987
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2114564/ https://ncbi.nlm.nih.gov/pubmed/3029142 |
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