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Assembly properties of fluorescein-labeled tubulin in vitro before and after fluorescence bleaching
Brain tubulin has been conjugated with dichlorotriazinyl- aminofluorescein (DTAF) to form a visualizable complex for the study of tubulin dynamics in living cells. By using several assays we confirm the finding of Keith et al. (Keith, C. H., J. R. Feramisco, and M. Shelanski, 1981, J. Cell Biol., 88...
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| Format: | Artigo |
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| Language: | Inglês |
| Published: |
The Rockefeller University Press
1984
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2113541/ https://ncbi.nlm.nih.gov/pubmed/6438113 |
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