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Membrane isolation on polylysine-coated beads. Plasma membrane from HeLa cells
HeLa cell plasma membranes have been purified after binding cells to polylysine-coated polyacrylamide beads. Cell attachment to beads and membrane recovery were maximal in a sucrose-acetate buffer, pH 5.0, at 25 degrees C. Measurements of ouabain-sensitive NaK-adenosine triphosphatase, membrane-boun...
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| Format: | Artigo |
|---|---|
| Jezik: | Inglês |
| Izdano: |
The Rockefeller University Press
1977
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2111560/ https://ncbi.nlm.nih.gov/pubmed/21192 |
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