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Rapid isolation of metaphase chromosomes containing high molecular weight DNA
Metaphase chromosomes with high molecular weight DNA were isolated from Chinese hamster ovary (CHO) cells in a neutral buffer containing polyamines and chelators. The individual, unfixed chromosomes retained their centromeric and secondary constrictions, distinct sister chromatids, and complex bandi...
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| Formato: | Artigo |
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| Idioma: | Inglês |
| Publicado em: |
The Rockefeller University Press
1979
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2111530/ https://ncbi.nlm.nih.gov/pubmed/39081 |
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