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Pitfalls in the use of lead nitrate for the histochemical demonstration of adenylate cyclase activity
The biochemistry of the lead histochemical technique for demonstrating adenylate cyclase was studied. The enzyme activity of fat cell plasma membranes, using 5'-adenylyl-imidodiphosphate (AMP-PNP) as substrate, was completely inhibited at 1 times 10- minus 4 M Pb(NO3)2 and yet at 4 times 10- mi...
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| Natura: | Artigo |
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| Lingua: | Inglês |
| Pubblicazione: |
The Rockefeller University Press
1975
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2111165/ https://ncbi.nlm.nih.gov/pubmed/165205 |
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