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Methods for the freeze-fracturing of nerve tissue cultures and cell monolayers

Two methods for freeze-cleaving of thin tissue layers are presented. Whereas a simple technique can be employed to fracture continuous, relatively firm tissue layers, a more sophisticated technique employing special carriers is needed to fracture very thin and incomplete layers, e.g., the fiber outg...

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Detalhes bibliográficos
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 1975
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2111159/
https://ncbi.nlm.nih.gov/pubmed/1127007
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