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Nucleation of microtubules in vitro by isolated spindle pole bodies of the yeast Saccharomyces cerevisiae
Spindle pole bodies (SPBs) were isolated from the yeast Saccharomyces cerevisiae by an adaptation of the Kleinschmidt monolayer technique. Spheroplasts prepared from the cells were lysed on an air-water interface. Spread preparations were picked up on grids, transferred to experimental test solution...
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| Natura: | Artigo |
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| Lingua: | Inglês |
| Pubblicazione: |
The Rockefeller University Press
1978
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2110130/ https://ncbi.nlm.nih.gov/pubmed/357437 |
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