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In vitro polymerization of microtubules into asters and spindles in homogenates of surf clam eggs
The eggs of the surf clam Spisula solidissima were artificially activated, homogenized at various times in cold 0.5 M MES buffer, 1mM EGTA at pH 6.5, and microtubule polymerization was induced by raising the temperature to 28 degrees C. In homogenates of unactivated eggs few microtubules form and no...
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| Format: | Artigo |
|---|---|
| Sprog: | Inglês |
| Udgivet: |
The Rockefeller University Press
1975
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2109483/ https://ncbi.nlm.nih.gov/pubmed/45844 |
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