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ISOLATION OF CILIATED OR UNCILIATED BASAL BODIES FROM THE RABBIT OVIDUCT

Techniques have been developed for the isolation of basal bodies with cilia attached or for the isolation of only basal bodies from the rabbit oviduct. Oviducts are removed, cut open, and placed in an extraction medium composed of 0.25 M sucrose, 0.001 M EDTA, 0.025 M KCl, 0.02 M Hepes buffer pH 7.5...

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Detalhes bibliográficos
Autor principal: Anderson, Richard G. W.
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 1974
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2109169/
https://ncbi.nlm.nih.gov/pubmed/4204973
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