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CYTOCHEMICAL LOCALIZATION OF 5'-NUCLEOTIDASE IN SUBCELLULAR FRACTIONS ISOLATED FROM RAT LIVER : I. The Origin of 5'-Nucleotidase Activity in Microsomes

A procedure has been developed for the cytochemical localization of 5'-nucleotidase in isolated, unfixed, rat liver microsomes. Membranes were incubated with adenosine 5'-phosphate (5'-AMP) and Pb(NO(3))(2) and then isolated on sucrose density gradients: all the phosphate released was...

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Hlavní autor: Widnell, Christopher C.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Rockefeller University Press 1972
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2108658/
https://ncbi.nlm.nih.gov/pubmed/5009518
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