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ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF NUCLEI FROM CHICK EMBRYO LIVER

A procedure is described for the isolation of enzymatically active nuclei from chick embryo liver. It consists of the homogenization of the pooled tissue in 0.32 M sucrose-3 mM MgCl(2) followed by a slow centrifugation. The resulting nuclear pellet is then purified further in a discontinuous density...

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Autors principals: Incefy, Genevieve S., Kappas, Attallah
Format: Artigo
Idioma:Inglês
Publicat: The Rockefeller University Press 1971
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2108274/
https://ncbi.nlm.nih.gov/pubmed/4398788
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