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PURIFICATION OF INTACT MICROTUBULES FROM BRAIN

Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay...

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Sonraí Bibleagrafaíochta
Main Authors: Kirkpatrick, Joel B., Hyams, Lyon, Thomas, Virginia L., Howley, Peter M.
Formáid: Artigo
Teanga:Inglês
Foilsithe: The Rockefeller University Press 1970
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2108086/
https://ncbi.nlm.nih.gov/pubmed/19866738
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