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COMBINED CYTOCHEMICAL AND ELECTRON MICROSCOPIC DEMONSTRATION OF β-GLUCURONIDASE ACTIVITY IN RAT LIVER WITH THE USE OF A SIMULTANEOUS COUPLING AZO DYE TECHNIQUE

Rat liver was fixed in formal-cacodylate-sucrose and frozen sections were incubated in a simultaneous-coupling medium containing naphthol AS-BI glucuronide as substrate and hexazonium pararosanilin as the diazo reagent. By light microscopy, the sections demonstrated β-glucuronidase activity as red d...

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Detalhes bibliográficos
Main Authors: Hayashi, Masando, Shirahama, Tsuranobu, Cohen, Alan S.
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 1968
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2107359/
https://ncbi.nlm.nih.gov/pubmed/4170545
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