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ISOLATION OF CELL NUCLEI FROM THE MAMMALIAN CEREBRAL CORTEX AND THEIR ASSORTMENT ON A MORPHOLOGICAL BASIS

An aqueous method is described for the isolation of highly purified nuclei from the cerebral cortex of adult guinea pigs. Erythrocytes were removed by a short-time perfusion of the brain, myelin fragments by a rapid mechanical method, and blood capillaries by a centrifugal sieving through dense sucr...

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Detalhes bibliográficos
Main Authors: Kato, Takahiko, Kurokawa, Masanori
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 1967
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2107272/
https://ncbi.nlm.nih.gov/pubmed/4226774
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