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THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER

Suspensions of isolated parenchymal cells were prepared from rat liver by incubation with collagenase and hyaluronidase followed by mechanical treatment. Utilization of 0.15% collagenase together with 0.15% hyaluronidase yielded adequate numbers of cells for experimental purposes. As shown by light...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Howard, Roger B., Christensen, A. Kent, Gibbs, Frederic A., Pesch, Leroy A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Rockefeller University Press 1967
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2107157/
https://ncbi.nlm.nih.gov/pubmed/4294245
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