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ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS

The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically inta...

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Autors principals: Huberman, Joel A., Attardi, Giuseppe
Format: Artigo
Idioma:Inglês
Publicat: The Rockefeller University Press 1966
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2107040/
https://ncbi.nlm.nih.gov/pubmed/5339564
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